AMINO ACID COMPOSITION OF FOUR RABBIT ANTIBODIES

Differences in the amino acid composition of two purified antibodies from the same rabbit.

Amino acid sequence of rabbit apolipoprotein E.

The complete amino acid sequence of rabbit apolipoprotein E (apoE) was determined by generating three sets of peptides using cyanogen bromide, endoproteinase AspN, and Staphylococcus aureus V8 protease to cleave the protein. Through twenty cycles of sequence analysis on the whole protein, glutamic acid was identified as the N-terminal residue of rabbit apoE; the C-terminus of the protein was id...

Rabbit testis proacrosin. Purification, molecular weight estimation, and amino acid and carbohydrate composition of the molecule.

Rabbit testis contains a single molecular form of proacrosin which could be extracted at acid pH and purified 145-fold by Sephadex G-100, SE-Sephadex, and concanavalin A-Sepharose affinity column chromatography. The purified proacrosin was judged to be homogeneous on the basis of chromatographic analyses, disc and sodium dodecyl sulfate-polyacrylamide disc gel electrophoresis, and immunodiffusi...

Amino acid composition of human plasma prothrombin.

The amino acid composition of purified human plasma prothrombin was determined by ion exchange chromatography. In some cases, the carbohydrate composition was also estimated. Seven preparations, isolated in two different laboratories and finally purified by two different methods, were studied. Within experimental error, all the preparations had the same amino acid composition, which was as foll...

Amino acid composition of hen's egg.

I . Pooled samples of eggs from White Leghorn and New Hampshire hens given diets containing 110 and zoo g protein/kg were analysed for their content of essential amino acids. 2. The amino acid composition of the hen’s egg protein was not significantly affected by variations in breed and diet. 3. The estimated values for lysine, total sulphur-containing amino acids and tryptophan in egg protein ...